When you tighten the axle nut it will affect the chain. Something that none of your friends have mentioned is that you need to find the "tight spot" on your chain. Due to imperfections in manufacturing and installation,you are bound to have a spot in the rotation of the rim where the chain is the tightest. You need to find that spot and take your measurements from that point. You should have about 3/4" at the tightest point,with the bike on the kickstand and the axle torqued.
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